Comparison of in vitro and in vivo bioassays to measure thyroid hormone disrupting activity in water extracts.
Identifieur interne : 000F39 ( Main/Exploration ); précédent : 000F38; suivant : 000F40Comparison of in vitro and in vivo bioassays to measure thyroid hormone disrupting activity in water extracts.
Auteurs : Frederic D L. Leusch [Australie] ; Natalie H. Aneck-Hahn [Afrique du Sud] ; Jo-Anne E. Cavanagh [Nouvelle-Zélande] ; David Du Pasquier [France] ; Timo Hamers [Pays-Bas] ; Armelle Hebert [France] ; Peta A. Neale [Australie] ; Marco Scheurer [Allemagne] ; Steven O. Simmons [États-Unis] ; Merijn Schriks [Pays-Bas]Source :
- Chemosphere [ 1879-1298 ] ; 2017.
Abstract
Environmental chemicals can induce thyroid disruption through a number of mechanisms including altered thyroid hormone biosynthesis and transport, as well as activation and inhibition of the thyroid receptor. In the current study six in vitro bioassays indicative of different mechanisms of thyroid disruption and one whole animal in vivo assay were applied to 9 model compounds and 4 different water samples (treated wastewater, surface water, drinking water and ultra-pure lab water; both unspiked and spiked with model compounds) to determine their ability to detect thyroid active compounds. Most assays correctly identified and quantified the model compounds as agonists or antagonists, with the reporter gene assays being the most sensitive. However, the reporter gene assays did not detect significant thyroid activity in any of the water samples, suggesting that activation or inhibition of the thyroid hormone receptor is not a relevant mode of action for thyroid endocrine disruptors in water. The thyroperoxidase (TPO) inhibition assay and transthyretin (TTR) displacement assay (FITC) detected activity in the surface water and treated wastewater samples, but more work is required to assess if this activity is a true measure of thyroid activity or matrix interference. The whole animal Xenopus Embryonic Thyroid Assay (XETA) detected some activity in the unspiked surface water and treated wastewater extracts, but not in unspiked drinking water, and appears to be a suitable assay to detect thyroid activity in environmental waters.
DOI: 10.1016/j.chemosphere.2017.10.109
PubMed: 29107228
Affiliations:
- Afrique du Sud, Allemagne, Australie, France, Nouvelle-Zélande, Pays-Bas, États-Unis
- Bade-Wurtemberg, District de Karlsruhe, Hollande-Septentrionale, Île-de-France
- Amsterdam, Karlsruhe, Maisons-Laffitte
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Leusch</name><affiliation wicri:level="1"><nlm:affiliation>Australian Rivers Institute, Griffith School of Environment, Griffith University, Southport, QLD, 4222, Australia. Electronic address: f.leusch@griffith.edu.au.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Australian Rivers Institute, Griffith School of Environment, Griffith University, Southport, QLD, 4222</wicri:regionArea><wicri:noRegion>4222</wicri:noRegion></affiliation></author><author><name sortKey="Aneck Hahn, Natalie H" sort="Aneck Hahn, Natalie H" uniqKey="Aneck Hahn N" first="Natalie H" last="Aneck-Hahn">Natalie H. Aneck-Hahn</name><affiliation wicri:level="1"><nlm:affiliation>Environmental Chemical Pollution and Health Research Unit, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa.</nlm:affiliation><country xml:lang="fr">Afrique du Sud</country><wicri:regionArea>Environmental Chemical Pollution and Health Research Unit, Faculty of Health Sciences, University of Pretoria, Pretoria</wicri:regionArea><wicri:noRegion>Pretoria</wicri:noRegion></affiliation></author><author><name sortKey="Cavanagh, Jo Anne E" sort="Cavanagh, Jo Anne E" uniqKey="Cavanagh J" first="Jo-Anne E" last="Cavanagh">Jo-Anne E. Cavanagh</name><affiliation wicri:level="1"><nlm:affiliation>Landcare Research, PO Box 69040, Lincoln, 7640, New Zealand.</nlm:affiliation><country xml:lang="fr">Nouvelle-Zélande</country><wicri:regionArea>Landcare Research, PO Box 69040, Lincoln, 7640</wicri:regionArea><wicri:noRegion>7640</wicri:noRegion></affiliation></author><author><name sortKey="Du Pasquier, David" sort="Du Pasquier, David" uniqKey="Du Pasquier D" first="David" last="Du Pasquier">David Du Pasquier</name><affiliation wicri:level="1"><nlm:affiliation>Laboratoire WatchFrog, 1 Rue Pierre Fontaine, 91000, Evry, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire WatchFrog, 1 Rue Pierre Fontaine, 91000, Evry</wicri:regionArea><wicri:noRegion>91000, Evry</wicri:noRegion><wicri:noRegion>91000, Evry</wicri:noRegion></affiliation></author><author><name sortKey="Hamers, Timo" sort="Hamers, Timo" uniqKey="Hamers T" first="Timo" last="Hamers">Timo Hamers</name><affiliation wicri:level="3"><nlm:affiliation>Vrije Universiteit Amsterdam, Department Environment & Health, De Boelelaan 1108, 1081 HZ, Amsterdam, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Vrije Universiteit Amsterdam, Department Environment & Health, De Boelelaan 1108, 1081 HZ, Amsterdam</wicri:regionArea><placeName><settlement type="city">Amsterdam</settlement><region nuts="2" type="province">Hollande-Septentrionale</region></placeName></affiliation></author><author><name sortKey="Hebert, Armelle" sort="Hebert, Armelle" uniqKey="Hebert A" first="Armelle" last="Hebert">Armelle Hebert</name><affiliation wicri:level="3"><nlm:affiliation>Veolia Research & Innovation, 78600, Maisons-Laffitte, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Veolia Research & Innovation, 78600, Maisons-Laffitte</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Maisons-Laffitte</settlement></placeName></affiliation></author><author><name sortKey="Neale, Peta A" sort="Neale, Peta A" uniqKey="Neale P" first="Peta A" last="Neale">Peta A. Neale</name><affiliation wicri:level="1"><nlm:affiliation>Australian Rivers Institute, Griffith School of Environment, Griffith University, Southport, QLD, 4222, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Australian Rivers Institute, Griffith School of Environment, Griffith University, Southport, QLD, 4222</wicri:regionArea><wicri:noRegion>4222</wicri:noRegion></affiliation></author><author><name sortKey="Scheurer, Marco" sort="Scheurer, Marco" uniqKey="Scheurer M" first="Marco" last="Scheurer">Marco Scheurer</name><affiliation wicri:level="3"><nlm:affiliation>DVGW - Technologiezentrum Wasser, Karlsruher Str.84, 76139, Karlsruhe, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>DVGW - Technologiezentrum Wasser, Karlsruher Str.84, 76139, Karlsruhe</wicri:regionArea><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Karlsruhe</region><settlement type="city">Karlsruhe</settlement></placeName></affiliation></author><author><name sortKey="Simmons, Steven O" sort="Simmons, Steven O" uniqKey="Simmons S" first="Steven O" last="Simmons">Steven O. Simmons</name><affiliation wicri:level="1"><nlm:affiliation>National Center for Computational Toxicology, Office of Research and Development, U.S. Environmental Protection Agency, Research Triangle Park, NC, 27711, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>National Center for Computational Toxicology, Office of Research and Development, U.S. Environmental Protection Agency, Research Triangle Park, NC, 27711</wicri:regionArea><wicri:noRegion>27711</wicri:noRegion></affiliation></author><author><name sortKey="Schriks, Merijn" sort="Schriks, Merijn" uniqKey="Schriks M" first="Merijn" last="Schriks">Merijn Schriks</name><affiliation wicri:level="1"><nlm:affiliation>KWR Watercycle Research Institute, Groningenhaven 7, 3433 PE, Nieuwegein, The Netherlands; Vitens Drinking Water Company, 8019 BE, Zwolle, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>KWR Watercycle Research Institute, Groningenhaven 7, 3433 PE, Nieuwegein, The Netherlands; Vitens Drinking Water Company, 8019 BE, Zwolle</wicri:regionArea><wicri:noRegion>Zwolle</wicri:noRegion></affiliation></author></analytic><series><title level="j">Chemosphere</title><idno type="eISSN">1879-1298</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Environmental chemicals can induce thyroid disruption through a number of mechanisms including altered thyroid hormone biosynthesis and transport, as well as activation and inhibition of the thyroid receptor. In the current study six in vitro bioassays indicative of different mechanisms of thyroid disruption and one whole animal in vivo assay were applied to 9 model compounds and 4 different water samples (treated wastewater, surface water, drinking water and ultra-pure lab water; both unspiked and spiked with model compounds) to determine their ability to detect thyroid active compounds. Most assays correctly identified and quantified the model compounds as agonists or antagonists, with the reporter gene assays being the most sensitive. However, the reporter gene assays did not detect significant thyroid activity in any of the water samples, suggesting that activation or inhibition of the thyroid hormone receptor is not a relevant mode of action for thyroid endocrine disruptors in water. The thyroperoxidase (TPO) inhibition assay and transthyretin (TTR) displacement assay (FITC) detected activity in the surface water and treated wastewater samples, but more work is required to assess if this activity is a true measure of thyroid activity or matrix interference. The whole animal Xenopus Embryonic Thyroid Assay (XETA) detected some activity in the unspiked surface water and treated wastewater extracts, but not in unspiked drinking water, and appears to be a suitable assay to detect thyroid activity in environmental waters.</div></front></TEI><affiliations><list><country><li>Afrique du Sud</li><li>Allemagne</li><li>Australie</li><li>France</li><li>Nouvelle-Zélande</li><li>Pays-Bas</li><li>États-Unis</li></country><region><li>Bade-Wurtemberg</li><li>District de Karlsruhe</li><li>Hollande-Septentrionale</li><li>Île-de-France</li></region><settlement><li>Amsterdam</li><li>Karlsruhe</li><li>Maisons-Laffitte</li></settlement></list><tree><country name="Australie"><noRegion><name sortKey="Leusch, Frederic D L" sort="Leusch, Frederic D L" uniqKey="Leusch F" first="Frederic D L" last="Leusch">Frederic D L. Leusch</name></noRegion><name sortKey="Neale, Peta A" sort="Neale, Peta A" uniqKey="Neale P" first="Peta A" last="Neale">Peta A. Neale</name></country><country name="Afrique du Sud"><noRegion><name sortKey="Aneck Hahn, Natalie H" sort="Aneck Hahn, Natalie H" uniqKey="Aneck Hahn N" first="Natalie H" last="Aneck-Hahn">Natalie H. Aneck-Hahn</name></noRegion></country><country name="Nouvelle-Zélande"><noRegion><name sortKey="Cavanagh, Jo Anne E" sort="Cavanagh, Jo Anne E" uniqKey="Cavanagh J" first="Jo-Anne E" last="Cavanagh">Jo-Anne E. Cavanagh</name></noRegion></country><country name="France"><noRegion><name sortKey="Du Pasquier, David" sort="Du Pasquier, David" uniqKey="Du Pasquier D" first="David" last="Du Pasquier">David Du Pasquier</name></noRegion><name sortKey="Hebert, Armelle" sort="Hebert, Armelle" uniqKey="Hebert A" first="Armelle" last="Hebert">Armelle Hebert</name></country><country name="Pays-Bas"><region name="Hollande-Septentrionale"><name sortKey="Hamers, Timo" sort="Hamers, Timo" uniqKey="Hamers T" first="Timo" last="Hamers">Timo Hamers</name></region><name sortKey="Schriks, Merijn" sort="Schriks, Merijn" uniqKey="Schriks M" first="Merijn" last="Schriks">Merijn Schriks</name></country><country name="Allemagne"><region name="Bade-Wurtemberg"><name sortKey="Scheurer, Marco" sort="Scheurer, Marco" uniqKey="Scheurer M" first="Marco" last="Scheurer">Marco Scheurer</name></region></country><country name="États-Unis"><noRegion><name sortKey="Simmons, Steven O" sort="Simmons, Steven O" uniqKey="Simmons S" first="Steven O" last="Simmons">Steven O. 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